O-167 The good egg: on the onset of transcription in mouse and human embryos

Abstract At the moment of union in fertilisation, sperm and oocyte are transcriptionally silent, but little is understood about how transcription initiates newly-formed embryo. In this talk, I shall suggest why fundamental process has remained arcane for so long, my lab sought to unlock its secrets mouse human embryos. By combining micromanipulation single-cell RNA-sequencing, we have revealed a programme embryonic initiation beginning within four hours fertilisation mouse. The talk will outline our efforts characterise initiating transcription, conservation one-cell Transcribed genes predict regulation by oncogenic factors, including c-Myc, whose features be detailed, together with model suggesting predictive parallels between onset embryogenesis cancer. Trial registration number XXXX